Determination of Content of Nutmeg Lignan and Dehydrodiisoeugenol in Nutmeg by HPLC
10.3870/yydb.2014.08.025
- VernacularTitle:高效液相色谱法同时测定肉豆蔻麸煨前后肉豆蔻木脂素和去氢二异丁香酚的含量
- Author:
Huan LIU
;
Jianfeng CHEN
;
Jing WANG
;
Zimin YUAN
- Publication Type:Journal Article
- Keywords:
Nutmeg;
Nutmeg lignan;
Dehydrodiisoeugenol;
Chromatograph,high performance liquid
- From:
Herald of Medicine
2014;(8):1070-1072
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develope a high performance liquid chromatograph ( HPLC ) method for simultaneous determination of nutmeg lignan and dehydrodiisoeugenol before and after processing of nutmeg. Methods The column was Diamonsil C18(250 mmí 4. 6 mm, 5 μm). The mobile phase was methanol-water in a gradient elution mode. The UV detection wave length was 274 nm. The column temperature was 25℃ . The flow rate was 1. 0 mL·min-1 . Results Nutmeg lignan and dehydrodiisoeugenol had a good linear correlation in ranges of 10. 24-61. 44 μg·mL-1(r=0. 999 6) and 3. 0-18. 0 μg·mL-1 (r=0.999 8), respectively. The average recovery rates were 97. 94% (RSD=2. 17%) and 97. 11% (RSD=2. 17%). Conclusion The method is simple, accurate, reproducible, and can be used for the simultaneous determination of nutmeg lignan and dehydrodiisoeugenol before and after the processing of nutmeg.
