Optimization of Extraction Technology for Total Saponins of Ardisia Crenata by Singlefactor Experiment Combined with Response Surface Methodology

VernacularTitle:单因素实验结合响应面法优化朱砂根总皂苷的提取工艺
Author: Qin WU ; Xun CHEN
Publication Type:Journal Article
Keywords: Ardisia crenata; Total saponins; Response surface methodology; Optimization of extraction
From: Herald of Medicine 2014;(8):1080-1082
CountryChina
Language:Chinese
Abstract: Objective To optimize the extraction process of Ardisia crenata based on the content of total saponins and to provide a basis for the Ardisia crenata study. Methods Based on the results of the single-factor tests and the Box-Behnken central composite experimental design principles, a response surface methodology which has three factors and three levels was designed to optimize the extraction process of Ardisia crenata based on the content of saponins. Results A maximal extraction yield of total saponins reached 2. 29% under the optimal conditions as follows:70% alcohol was used as extraction solvent with the material to liquid ratio of 116, the extraction time was 3 h at 70 ℃. Conclusion The optimized extraction process is accurate, reliable and practically valuable.