Simultaneous Content Determination of Hesperidin and Phillyrin in Bao-He-Wan by HPCE

VernacularTitle:高效毛细管电泳法同时测定保和丸中橙皮苷与连翘苷的含量
Author: Chaoyun ZHANG ; Pengfei HAO ; Xianrong XI
Publication Type:Journal Article
Keywords: Bao-He-Wan; HPCE; hesperidin; phillyrin
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(7):1668-1671
CountryChina
Language:Chinese
Abstract: This study was aimed to establish a HPCE method for the content determination of hesperidin and phillyrin in Bao-He-W an (BHW). Fused silica capillary (75 cm í 50 μm) was employed and 30 mmol·L-1 borax so-lution (8% acetonitrile, pH9.64) was served as the running buffer. Other conditions were as follows: electrokinetic injection was 50 kPa í 20 s; analytical voltage was 20 kV; temperature was 20℃; and detection wavelength was 254 nm. The silica capillary was flushed with 0.1 mol·L-1 sodium hydrate and the running buffer for 10 min before each injection, respectively. The results showed that the linearity of hesperidin was in the range of 0.10~2.40 mg·mL-1 (r=0.999 4), the average recovery was 99.85% and RSD=2.34%. The phillyrin was in the range of 0.07~0.84 mg·mL-1 (r=0.999 2), the average recovery was 99.16% and RSD=2.78%. It was concluded that the method was rapid and sensitive. It can be used for the quality control of content determination of hesperidin and phillyrin in BHW.