Preparation of Label-free Thrombin Aptasensor Based on Pd-Au Nanoparticles
10.11895/j.issn.0253-3820.140037
- VernacularTitle:基于Pd-Au纳米颗粒的非标记型凝血酶传感器的研制
- Author:
Yajuan FENG
;
Yunhui YANG
- Publication Type:Journal Article
- Keywords:
Label-free;
Aptasensor;
Thrombin;
Palladium-gold nanoparticles
- From:
Chinese Journal of Analytical Chemistry
2014;(8):1138-1143
- CountryChina
- Language:Chinese
-
Abstract:
Pd nanoparticles (Pd NPs) were electrodeposited on the glassy carbon electrode (GCE). Then, Pd NPs/GCE was further inserted into H2 SO4 solution to polarize for 5 min to absorb a certain amount of active hydrogen. Then, the electrode was quickly inserted to HAuCl4 solution for 15 min. AuNPs were deposited spontaneously on the surface of Pd NPs due to the reduction of active hydrogen. As a result, Pd-AuNPs were modified on the surface of GCE. Next, aptamer I of thrombin was immobilized on Pd-Au nanoparticles. In the presence of thrombin, it bound with the aptamer immobilized on Pd-Au nanoparticles and thus the formed complex obstructed the catalysis of Pd-Au nanoparticles to H2 O2 . Hence, the reduction current of H2 O2 decreased with the increase of thrombin concentration. The aptamer sensor had a good linear relationship with the concentration of thrombin in the range of 2. 98-297 nmol/L with a detection limit of 0. 98 nmol/L.
