Acute Toxicity Test and Diuresis Effect of Esculentoside A

VernacularTitle:商陆皂苷甲急性毒性与利尿作用研究
Author: Nannan CUI ; Xianglong MENG ; Junnan MA ; Kun LI ; Shuosheng ZHANG
Publication Type:Journal Article
Keywords: Esculentoside A; Toxicity,acute; Diuresis effect
From: Herald of Medicine 2014;(8):981-984
CountryChina
Language:Chinese
Abstract: Objective To understand the intensity and characteristics of acute toxicity of esculentoside A on mice and measure relevant parameters and observe its diuresis effect on rat. Methods After intraperitoneal injection of different concentrations of esculentoside A to mice, toxic reactions were observed. Rats with water load were intraperitoneally injected with different doses of esculentoside A. Total urine volume in six consecutive hours after the injection was determined. Results The LD50 of esculentoside A calculated by Bliss method was 26. 19 mg · kg-1 , and the 95% confidence interval was 23. 11-29. 85 mg·kg-1 . The mortality and acute toxicity of esculentoside A appeared to be dose-dependent while the blank control group had no abnormal reaction. The urine volume was significantly different between high dose group and the negative control group. No significant difference in urine volume was found between middle and the negative control group, and between low dose group and the negative control group. Conclusion Esculentoside A is poisonous to mice when single dose was intraperitoneally injected, and high dose of esculentoside A has diuresis effect on rat.