Transcriptomic Analysis and Systematic Mining of Genes Involved in Biosynthetic Pathway of Triterpenoid Saponins in Ilex Asprella
10.11842/wst.2014.07.011
- VernacularTitle:岗梅转录组及其乌索烷型三萜皂苷生物合成相关酶基因的发掘
- Author:
Xiasheng ZHENG
;
Xiuxiu LUO
;
Hui XU
;
Ruoting ZHAN
;
Weiwen CHEN
- Publication Type:Journal Article
- Keywords:
Ilex asprella;
transcriptome;
triterpenoid saponin;
biosynthesis;
gene mining
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(7):1505-1512
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to screen candidate genes involved in the triterpenoid saponins biosynthetic pathway of the Ilex asprella root. The Illumina platform was applied to perform transcriptomic sequencing of I. asprella root, followed by a series of bioinformatics analysis. The results showed that a total of 272 candidate unigenes were anno-tated to be involved in the biosynthetic pathway of terpenoid in the transcriptome of I. asprella root, including 72 u-nigenes for the upstream pathway and 26 unigenes for cyclization, oxidation and glycosylation in the downstream pathway. Phylogenetic analysis was carried out to further analyze the evolution relationship of some candidate uni-genes and their homologous genes. Two genes IaA S1 and IaA S2 were proved to be mixed amyrin synthases in yeast expression system. Moreover, IaA S1 was identified to one of the rare ASs with α-amyrin as the major product. It was concluded that a series of candidate genes, which might be involved in the biosynthetic pathway of triterpenoid saponins, were screened out from the transcriptome of I. asprella root. Further investigation of these candidate genes will provide insight into their actual functions in the triterpenoid saponins biosynthetic pathway in I. asprella.
