Salmonella typhimurium Flagellin as adjuvants can enhance immune response of DnaJ protein
10.3969/j.issn.1000-484X.2014.08.004
- VernacularTitle:鼠伤寒沙门氏菌鞭毛蛋白可增强DnaJ蛋白的免疫反应
- Author:
Ming YAN
;
Yusi LIU
;
Shuai ZHANG
;
Wenchun XU
;
Hong WANG
;
Xuemei ZHANG
- Publication Type:Journal Article
- Keywords:
Flagellin;
DnaJ protein;
Streptococcus pneumonia;
Adjuvant
- From:
Chinese Journal of Immunology
2014;(8):1023-1027
- CountryChina
- Language:Chinese
-
Abstract:
To make sure the role of flagellin as mucosal adjuvants and the protective effect of streptococcus pneumonia infection when combined with DnaJ protein.Methods:Recombinant plasmid pET-28 ( a)/flagella was transferred to E.coli BL21(DE3).Over-expression of flagella was induced by IPTG and purification for animal study.All the C57BL/6 mice were randomly divided into three groups ,then respectively intranasally given the mixture of flagellin and DnaJ protein ( experimental group ) , DnaJ protein( control group 1 ) and the mixture of GST and DnaJ protein ( control group 2 ).The serum IgG and its subtype , cytokines secreted by mice spleen cells were detected by ELISA.At last all the C57BL/6 mice were intranasally immunized with Streptococcus pneumoniaD39.The protective effect by survival times were evaluated.Results: The mice of experimental group could secrete high level of serum IgG and cytokines IFN-γ,IL-4 and IL-17A.What more,the survival rate of mice in experimental group was 60%,a significant statistical difference with the control group.Conclusion:The flagellin as an adjuvant can reinforce the immune response of DnaJ protein and have better protection of resistance D 39 infection.We suggest that flagellin can be used as protein vaccine adjuvants.
