Regulating Effects of Shen-Xiong Hua-Yu Capsule Preconditioning on Expression Subtype of NOS in Cerebral Ischemia-reperfusion Injury among Rats
10.11842/wst.2014.07.017
- VernacularTitle:参芎化瘀胶囊预处理对大鼠脑缺血再灌注损伤后一氧化氮合酶亚型表达的影响
- Author:
Xiaoya CUI
;
Bin LIU
;
Xiaoyan CHEN
;
Yongwei LUO
;
Yuchao MIAO
;
Xinyu WANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia-reperfusion injury;
nitric oxide synthase;
endothelial nitric oxide synthase;
nervous nitric oxide synthase;
inducible nitric oxide synthase;
Shen-Xiong Hua-Y u capsule;
rat model
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(7):1552-1557
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the regulating effect of Shen-Xiong Hua-Y u (SXHY) capsule precondi-tioning on the expression of subtypes of nitric oxide synthase (NOS), including endothelial nitric oxide synthase (eNOS), nervous nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), in cerebral ischemia-reperfu-sion injury (CIRI) among rats, and to further clarify the mechanism of protective effect by SXHY capsule on acute CIRI rats. Rats were randomly divided into the sham operation group, CIRI group, and SXHY capsule of high-, medium-, and low-dose preconditioning group (480, 240, 120 mg·kg-1). Each group was further randomly divided into different subgroups, which were the 3, 6, 12, 24, 48, 72 h group after 2 h CIRI (n=6). Intragastric administration was given once a day for 7 days. The middle cerebral artery occlusion (MCAO) and reperfusion model was repro-duced by an intraluminal filament method on the 7th day. The protein expressions of eNOS, nNOS and iNOS were measured by immunhistochemical method. The results showed that compared with the sham operation group, expres-sions of eNOS, nNOS and iNOS in the CIRI group were increased at different time points (i.e., 3, 6, 12, 24, 48, 72 h, P< 0.05 or P< 0.01). Compared with the CIRI group, eNOS expression increased at different time points in SX-HY capsule group (P< 0.05 or P< 0.01). The nNOS and iNOS expression decreased at different time points (P<0.05 or P < 0.01). Among them, the high-dose group was the group with the most obvious effect. It was concluded that SXHY capsule preconditioning had protective effect on CIRI. Its mechanism may be related to the regulation on protein expression of NOS subtypes.
