Effect of salidroside on activity of endothelial progenitor cells and phos-phoinositide 3-kinase/Akt signaling pathway
10.3969/j.issn.1000-4718.2014.07.007
- VernacularTitle:红景天苷对内皮祖细胞功能及其 PI3 K／Akt 通路的影响
- Author:
Zhilan ZHOU
;
Linjing WANG
;
Gexiu LIU
;
Jincan ZHU
;
Xiaoyu CHEN
;
Shantao LIU
- Publication Type:Journal Article
- Keywords:
Salidroside;
Endothelial progenitor cells;
Phosphoinositide 3-kinase/Akt signaling pathway
- From:
Chinese Journal of Pathophysiology
2014;(7):1192-1195
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether salidroside has influence on the activities of endothelial progenitor cells (EPCs) and its mechanism.METHODS:Mononuclear cells from normal human peripheral blood were cultured in fi-bronectin coated flasks in endothelial progenitor medium .After 7 d, EPCs were characterized as adherent cells with acLDL-DiI uptaking and lectin binding by direct fluorescent staining .The proliferation and migration of EPCs were analyzed by MTT assay and Transwell chamber assay , respectively.The EPCs adhesion assay was performed by re-plating the cells on fibronectin-coated dishes , and then adherent cells were counted .NO and Akt protein were also detected .RESULTS:Sali-droside promoted EPCs proliferative , migratory and adhesive capacities in a concentration dependent manner .Salidroside also increased NO secretion , and the level of phosphorylated Akt protein .However , the effects of salidroside on EPCs were inhibited by phosphoinositide 3-kinase inhibitor LY294002.CONCLUSION:Salidroside regulates the activity of EPCs by phosphoinositide 3-kinase/Akt signaling pathway .
