RASSF1A expression mediated by lentivirus inhibits growth of small-cell lung cancer cell line H446
10.3969/j.issn.1000-4718.2014.07.010
- VernacularTitle:慢病毒介导的 RASSF1A 表达抑制小细胞肺癌 H446细胞的生长
- Author:
Lijun KONG
;
Lixia ZHANG
;
Xiying LUAN
;
Lisha ZHANG
;
Xuhan WANG
;
Hengyun YU
;
Yuezhi ZHANG
- Publication Type:Journal Article
- Keywords:
Ras-association domain family 1A;
Lentivirus;
Lung neoplasms;
Cell cycle
- From:
Chinese Journal of Pathophysiology
2014;(7):1209-1213
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the inhibitory effect of Ras-association domain family 1A ( RASSF1A) on the small-cell lung cancer cell growth .METHODS:The lentiviral expression vector containing RASSF1A gene was constructed and used to infect the small-cell lung cell line H446.The growth curve and cell cycle were detected by MTT assay and flow cytometry.The mRNA and protein levels of cell cycle-associated proteins were determined by real-time PCR and Western blotting.RESULTS:We obtained the H446 cells in which RASSF1A was stably expressed (named RASSF1A-H446). Compared with normal cell group and negative cell group , RASSF1A inhibited the proliferation of H446 cells, and arrested H446 cells in G1 phase.The expression of p21 and p27 was significantly increased , and E2F1 was significantly decreased in RASSF1A-H446 cells.CONCLUSION:RASSF1A inhibits the H446 cell growth by increasing the expressions of p 21 and p27, and decreasing the expression of E 2F1.
