Injection stauntoniae inhibits chronic inflammatory pain responses by changing COX-2 expression in carrageenan-inflamed mice
10.3969/j.issn.1000-4718.2014.07.011
- VernacularTitle:野木瓜注射液对炎性疼痛小鼠背根神经节CO X-2表达的影响
- Author:
Yanjing YANG
;
Xiaojun LI
;
Liwei LIU
;
Qiang GUO
;
Yusang LI
;
Lijing FENG
;
Hebin TANG
- Publication Type:Journal Article
- Keywords:
Anti-inflammation;
Analgesia;
Injection stauntoniae;
Cyclooxygenase 2;
Dorsal root ganglion
- From:
Chinese Journal of Pathophysiology
2014;(7):1214-1217
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of injection stauntoniae ( IS) on inflammatory pain responses in mice.METHODS:The carrageenan test was used to determine the anti-inflammatory and analgesic effects of IS .Except for control group, the mice in other groups received an injection of λ-carrageenan solution (1%, 50 μL) into the plantar region of the left hind paws , followed by a subcutaneous injection of IS at doses of 12.5%, 50%and 100%or equal vol-ume of 0.9%NaCl.Both paw edema and hyperalgesia to thermal stimulation were measured 4 h, 12 h, 24 h and 48 h after the injection of λ-carrageenan solution.The lumbar-5 (L5) dorsal root ganglions (DRGs) of the mice were taken to inves-tigate the cyclooxygenase 2 ( COX-2) expression by immunohistochemical staining .RESULTS:Subcutaneous injection of IS potently inhibited paw edema and hyperalgesia induced by λ-carrageenan in the mice accompanied with the inhibition of COX-2 protein expression in L 5 DRGs.CONCLUSION:IS exerts the anti-inflammatory and analgesic effects on the in-flammatory responses by inhibiting the protein expression of COX-2 in DRGs.
