The mechanism of ultrasound-target microbubble destruction for accelerating neovascularisation in ischemic skeletal muscle
10.3969/j.issn.1006-5725.2014.15.005
- VernacularTitle:超声空化微泡效应促进缺血骨骼肌血管新生的机制
- Author:
Yuanyuan BAI
;
Jiancheng XIU
;
Yili LIU
- Publication Type:Journal Article
- Keywords:
Ultrasound;
Microbubble;
Angiogenesis;
Inflammation
- From:
The Journal of Practical Medicine
2014;(15):2367-2370
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and possible mechanism of ultrasound-targeted microbubble destruction for accelerating neovascularisation in ischemic skeletal muscle. Methods Unilateral hind limb ischaemia was surgically induced in thirty wister rats. On postoperative day 7 , the rats were randomly divided into three groups: ultrasound-targeted microbubble destruction group (group A), ultrasound group (group B), and control group. After the end of the experiment , parafin sections for the skeletal muscle from one rat in each group were made to observe the changes in microstructure. The remaining rats were sacrificed at 24 h and on day 7. VEGF expression, inflammatory factor E-selectin, and monocyte chemotactic factor-1 (MCP-1) were detected in the rats. Results As compared with the other two goups, expressions of VEGF, neovascularization, E-selectin, and MCP-1 in the skeletal muscle were significantly increased in group A. Conclusions Microvascular rupture caused by ultrasound-targeted microbubble destruction can promote angiogenesis by stimulating secretion of endogenous VEGF in skeletal muscle; meanwhile, an increase in expression level of inflammatory factors may be one of the mechanisms.
