Identification of Original Plants of Guanzhong (Dryopteris Species) by Chloroplast psbA-trnH Intergenic Region

VernacularTitle:基于序列的鳞毛蕨属贯众药材基原植物的鉴定研究
Author: Feng LIU ; Yanfengyang JIANG ; Ruoting ZHAN ; Xinye MA ; Weiwen CHEN
Publication Type:Journal Article
Keywords: Guanzhong; Dryopteri; DNA barcodes; psbA-trnH
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(7):1482-1486
CountryChina
Language:Chinese
Abstract: This article was aimed to study the species identification capability of psbA-trnH among Guanzhong herbs from Dryopteri. The nucleotide sequence information of psbA-trnH region was abstracted using standardized manners from 9 Dryopteri species (19 samples). The identification efficiency of psbA-trnH was analyzed based on TaxonGap among both tested materials (9 species, 19 samples) and tested materials plus GenBank data (17 species, 44 samples in total). The results showed that with the expanded species range, the discriminative efficiency of psbA-trnH de-clined from 100% (9/9) to 82.4% (14/17), while the proportion of within-specific heterogeneity larger than between-specific separability increased from 11.1% (1/9) to 52.9% (9/17). It was concluded that psbA-trnH can be recom-mended as the valuable barcode for homonym distinguishment among Guanzhong herbs, with attention of adequate sampling within species.