Quality Analysis of Three Components in Shuanghuanglian Powder for Injection Before and After Ultrasonic Atomization
10.3870/yydb.2014.08.026
- VernacularTitle:双黄连粉针剂超声雾化前后3种成分的质量分析
- Author:
Fei HAN
;
Xiong XIAO
;
Qing WANG
;
Shicheng CHEN
;
Xiaojian LUO
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian powder injection;
Chlorogenic acid;
Forsythin;
Baicalin;
Ultrasonic,atomizetion;
Inhalation,aerosol
- From:
Herald of Medicine
2014;(8):1073-1076
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new RP-HPLC method for simultaneous determination of chlorogenic acid,forsythin, and baicalin in shuanghuanglian powder for injection after ultrasonic atomization. Methods Hypersil ODS2 C18(250 mmí4. 6 mm,5 μm) was used as the chromatographic column. The mobile phase was methanol-0. 2% phosphate acid solution (4060). Flow rate was 1. 0 mL·min-1 . Sample volume was 5μL. Column temperature was 30℃. Detection wavelength was 324 nm at 0-10 min and 277 nm at 10-25 min. Results Contents of chlorogenic acid,forsythin, and baicalin had good linear relationship with the respective peak area (r≥0. 999 7) within the scope of the sample volume. The RSD was <2% for precision, reproducibility, and stability. Recovery rate was 98. 50%-101. 12% (n=6). Conclusion The method is rapid, accurate and reproducible, with high resolution. It can determine the content of three kinds of components at the same time. The three components in shuanghuanglian powder for injection did not change significantly before and after ultrasonic atomization.
