Preparation and evaluation of flutide-loaded PLGA nanoparticles modified with cell-penetrating peptides
10.7644/j.issn.1674-9960.2014.07.012
- VernacularTitle:透膜肽修饰的载流感泰得PLGA纳米粒的制备与评价
- Author:
Qingbo XU
;
Xiaoran DING
;
Lina DU
;
Dandan LU
;
Yiguang JIN
;
Shengqi WANG
- Publication Type:Journal Article
- Keywords:
nanoparticles;
cell-penetrating peptides;
PLGA;
oligodeoxynucleotide;
sustained release
- From:
Military Medical Sciences
2014;(7):518-522
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare and evaluate flutide-loaded PLGA nanoparticles modified with cell-penetrating peptide-TAT.Methods The sequence of TAT was synthesized with florenl methyoxycarbonyl amino acids .The purity and molecular weight of TAT were determined using RP-HPLC and MALDI-TOF-MS.PLGA was modified with the TAT peptide and then prepared into flutide-loaded nanoparticles ( TAT-PLGA NPs) with the double emulsion method .The physical and chemical properties were evaluated , including size distribution, Zeta potential, SEM of nanoparticles , loading ratio of drug content and release profiles of TAT-PLGA NPs in vitro.The cytotoxicity of TAT-PLGA NPs was evaluated by CCK-8 methods.Results The purity of synthesized TAT was 95.6%, and molecular weight was 1495.8.The mean diameter,Zeta potential, drug loading ratio of TAT-PLGA nanoparticals were (159.5 ±2.1) nm, -(1.87 ±0.6) mV, and (5.75 ±0.17)μg/mg, respectively.The nanoparticles observed by transmission electron microscopy (TEM) had a spherical shape and uniform size without aggregation .In vitro release test showed sustained release of flutide from TAT-PLGA nanoparticles .Cell proliferation assay revealed that the TAT-PLGA nanoparticles did not damage the cell growth in vitro and showed good compatibility.Conclusion TAT-PLGA nanoparticles are prepared successfully by double emulsion method,and have sustained-release effect and good compatibility in vitro.They have potential application prospect in prevention and treatment of influenza .
