A high-fidelity DNA unidirection synthesis method:preliminary exploration
10.7644/j.issn.1674-9960.2014.07.014
- VernacularTitle:一种高保真单向DNA合成方法初步探索
- Author:
Xun WU
;
Yuxia LI
;
Beiping LI
;
Bingjuan LI
;
Dongmei BAI
;
Xin ZHANG
;
Yan LING
;
Wei ZHOU
;
Gang LIU
;
Huipeng CHEN
- Publication Type:Journal Article
- Keywords:
DNA fragment synthesis;
unidirection stretch;
high fidelity DNA unidirection synthesis method
- From:
Military Medical Sciences
2014;(7):527-531
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple but quick method to improve the high fidelity of the synthesis of DNA frag -ments.Methods High fidelity DNA unidirection synthesis method (HFUS) was presented and used that involved Phusion DNA polymerase, BsrDⅠrestriction enzymes and λexonuclease.Using the same system at different temperatures , HFUS method synthesized one positive single-strand DNA and several reverse single -stranded DNA one by one into the target DNA fragment.Results Two random sequences DNA fragments of 340 bp and 450 bp were synthesized using HFUS meth-od.Conclusion This article explores a new method for the synthesis of genes .Through the harvest of 450 bp DNA, HFUS may promise to be a new approach to the synthesis of DNA .
