NaF· EDTA-K2 negatively bias blood lipids and lipoproteins measurement in pregnant women
10.3760/cma.j.issn.1009-9158.2014.09.016
- VernacularTitle:NaF · EDTA-K2抗凝对妊娠妇女血脂分析的影响
- Author:
Yumei DAI
;
Yaoxiang LONG
;
Xiantao QIU
;
Xiaoqiong GU
;
Qihua LIANG
;
Xiaojuan CHEN
;
Minmin CAI
- Publication Type:Journal Article
- Keywords:
Pregnant women;
Lipids;
Laboratory techniques and procedures;
Edetic acid;
Anticoagulants
- From:
Chinese Journal of Laboratory Medicine
2014;(9):705-708
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of NaF · EDTA-K2 on blood lipids measurement in pregnant women , determine whetherthe empty-stomach plasma for OGTT could be used as substitute for serumin blood lipids analyses.Methods Fastingplasma with NaF · EDTA-K2 and serumfrom 100 pregnant women werecollected,andconcentration of CHO,TG, HDL,LDL,apoA1 and apoB were tested with Hitachi 7 600 automatic biochemical analyzer.Data were analyzed by SPSS 19.0 software.Results The concentrations oflipids and lipoproteins with NaF · EDTA-K2 were slightly lower than those of serum.There were statistical significances between the means of CHO , HDL, LDL, apoA1 and apoBof two groups ( P<0.05) except TG.Correlation coefficients of the six analyteswere CHO 0.968, TG 0.995, HDL 0.979, LDL 0.991, apoA1 0.692, apoB 0.846respectively.Standard Error of Estimate for plasma were: CHO 0.281, TG 0.094, HDL 0.077, LDL 0.112, apoA1 0.230, apoB 0.111 respectively.The modified coefficients were 1.08 for CHO, HDL and LDL, 1.14 for apoA1 and 1.07 for apoB.Conclusion NaF· EDTA-K2 negatively biased the blood lipidsmeasurement of CHO , HDL, LDL, apoA1 and apoB in pregnant women,yet lipids concentrations in plasma with NaF · EDTA-K2 were closely related to those in serum , which may be used to predict the bloodlipidslevel of pregnant women afterajusted.
