Effect of bisphosphonate on osteoclast differentiation and tartrate-resistant acid phosphatase
10.3969/j.issn.2095-4344.2014.38.001
- VernacularTitle:双膦酸盐对破骨细胞分化及抗酒石酸酸性磷酸酶的影响
- Author:
Wei DONG
;
Xiaojie FENG
;
Yongqiang LIANG
;
Jiupeng DENG
;
Liming WEN
;
Mengchun QI
- Publication Type:Journal Article
- Keywords:
tissue engineering;
diphosphonates;
osteoclasts;
osteoporosis
- From:
Chinese Journal of Tissue Engineering Research
2014;(38):6069-6073
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Tartrate-resistant acid phosphatase is a specific marker for osteoclast differentiation and bone resorption, which is a sign of osteoclast maturity.
OBJECTIVE:To study the effect of alendronate on tartrate-resistant acid phosphatase related to osteoclast differentiation and bone resorption.
METHODOsteoclasts were cultured by mouse monocyte-macrophage cellline-RAW264.7. The cells were divided into two groupcontrol group, treated with 100μg/L receptor activator of nuclear factorκB ligand factor;alendronate group, treated with 100μg/L receptor activator of nuclear factorκB ligand factor+10-7 mol/L alendronate. Osteoclastogenesis and resorption function of osteoclasts were examined at 7 days of culture. Gene expression of tartrate-resistant acid phosphatase was detected by immunofluorescence method. Western blot assay was used to detect protein expression of tartrate-resistant acid phosphatase.
RESULTS AND CONCLUSION:Tartrate-resistant acid phosphatase positive multinuclear cells were observed and resorption lacunae formed in two groups. Control group showed the higher number of tartrate-resistant acid phosphatase positive multinuclear cells and larger size of resorption lacunae than the alendronate group (P<0.01). Immunofluorescence showed expression of tartrate-resistant acid phosphatase was higher in the control group than the alendronate group (P<0.01);furthermore, the protein expression of tartrate-resistant acid phosphatase was also lower in the alendronate group than the control group (P<0.01). These findings indicate that bisphosphonates could strongly inhibit osteoclastogenesis and its resorption function by inhibiting protein expression of tartrate-resistant acid phosphatase.
