Bone morphogenetic protein-4 compounded with platelet-rich plasma promotes bone healing
10.3969/j.issn.2095-4344.2014.38.006
- VernacularTitle:富血小板血浆协同骨形态发生蛋白4促进骨愈合的效应
- Author:
Jianjie SHI
;
Zhibin LUO
;
Wenxiong CHEN
- Publication Type:Journal Article
- Keywords:
maxil a;
bone morphogenetic proteins;
platelet-rich plasma;
calcium phosphates
- From:
Chinese Journal of Tissue Engineering Research
2014;(38):6099-6109
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have shown that bone morphogenetic proteins play a key role in skeletal development. Platelet-rich plasma alone in animal or clinical trials cannot significantly promote bone graft healing.
OBJECTIVE:To investigate the osteogenesis effect of bone morphogenetic protein-4 compounded with platelet-rich plasma the bone defect area.
METHODTwenty-four New Zealand white rabbits were selected to establish maxil ary bone defect models, and then were randomly divided into four groups, six rats in each group. Group A was blank control group;Group B wasβ-tricalcium phosphate (0.1 g)+Bio-gide group;group C wasβ-tricalcium phosphate (0.1 g)+Bio-gide+platelet-rich plasma (1 mL) group;and group D wasβ-tricalcium phosphate (0.1 g)+Bio-gide+platelet-rich plasma (1 mL)+bone morphogenetic protein-4 (5μg). Gross observation, histological observation and imaging analysis were performed for analysis of new bone formation at weeks 4, 8, 12 after modeling.
RESULTS AND CONCLUSION:After 4 weeks, group D had more new bone and vessels formed in the bone defect area than the group B (P<0.01);however, the amount of new bone was higher in the group B than the group A (P<0.01). After 4, 8, 12 weeks, the amount of new bone in the bone defect area was higher in the group D than the groups B and C (P<0.01), and lowest in the group A (P<0.01). In theβ-tricalcium phosphate scaffold, platelet-rich plasma combined with bone morphogenetic protein can significantly promote the healing of bone defects.
