Determination of 4-Hydroxy-3-Methoxy Benzoic Acid by RP-HPLC and Inhibitory Effectof Phloroglucinol on Catecholamine-O-Methyl Transferase
10.3870/yydb.2014.10.012
- VernacularTitle:反相高效液相色谱法测定4-羟基-3-甲氧基苯甲酸及间苯三酚对儿茶酚氧位甲基转移酶的抑制作用
- Author:
Ruiling YANG
;
Hong LIU
;
Qi WANG
;
Kefei JI
;
Mengjiao SONG
- Publication Type:Journal Article
- Keywords:
Phloroglucinol;
Catecholamine-O-methyl transferase;
HPLC;
Inhibitory effect
- From:
Herald of Medicine
2014;(10):1303-1306
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect content of 4-hydroxy-3-methoxy benzoic acid by RP-HPLC and observe the inhibitory effect of phloroglucinol on catecholamine-O-methyl transferase (COMT). Methods This study used the principle of 3,4-dihydroxy benzoic acid transforming to 4-hydroxy-3-methoxy benzoic acid under COMT’ s catalytic action. COMT (20 μL) was extracted from mouse liver homogenate. In a reaction system,10 μL catecol (1í10-3 mol·L-1 ) and 10 μL phloroglucinol (5í10-3 , 1í10-3 and 2í10-4 mol·L-1 ,respectively) were added. Products were determined by RP-HPLC to analyze effects of 4-hydroxy-3-methoxy benzoic acid on COMT. Results Phloroglucinol had inhibitory effect on COMT activity at concentrations of 5í10-3 mol·L-1 ,1í10-3 mol·L-1 and 2 í10-4 mol ·L-1 ,with the inhibition rate being 25. 3% ,17. 6% and 8. 9% ,respectively. Conclusion Phloroglucinol has an inhibitory effect on COMT activity,which is weaker than the effect of catechol of the same concentration.
