HPLC Fingerprint Analysis of Wuzi Yanzong Pills
10.3870/yydb.2014.10.029
- VernacularTitle:五子衍宗丸高效液相色谱指纹图谱研究
- Author:
Wei LIU
;
Jianliang ZHOU
;
Bilian CHEN
;
Ming ZHU
- Publication Type:Journal Article
- Keywords:
Wuzi yanzong pills;
Fingerprint;
Chromatography,high performance liquid
- From:
Herald of Medicine
2014;(10):1360-1364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the high performance liquid chromatography(HPLC) fingerprint of wuzi yanzong pills. Methods HPLC was performed on Agilent Extend C18 column (250 mmí4. 6 mm,5 μm) with a gradient elution system using acetonitrile:methanol (101)-0. 4% phosphoric acid as the mobile phase. The column temperature was set at 30 ℃ and the flow rate was 1. 0 mL·min-1 . The eluate was detected at the wavelength of 254 nm. Chromatographic peaks were identified by LC-MS method. Results Nine common peaks in wuzi yanzong pill samples were identified by comparing their LC-MS data with those of reference compounds and related reference reports. The HPLC fingerprint of wuzi yanzong pills was finally developed based on the analysis of sixteen batches of samples and their similarities were above 0. 93. Conclusion This method has high precision,stability and repeatability. This study could be used for overall quality assessment of wuzi yanzong pills.
