Quantitative Determination of Sildenafil in Aphrodisiacs by LC-MS
10.3870/yydb.2014.10.032
- VernacularTitle:液相色谱-质谱联用法检测壮阳类中成药中西地那非的含量
- Author:
Yinhua DENG
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Sildenafil;
Liquid chromatography-mass spectrometry;
Multiple reaction monitoring
- From:
Herald of Medicine
2014;(10):1373-1375
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the quantitative determination of sildenafil in aphrodisiacs by liquid chromatography-mass spectrometry (LC-MS). Methods Chromatography was carried on a Waters BEH C18 column (50 mmí2. 1 mm,1. 7 μm) with the column temperature being 35 ℃ and 0. 02 mol·L-1 ammonium acetate water solution (containing 0. 1% acetic acid)-acetonitril (7030) as the mobile phase at a flow rate of 0. 2 mL·min-1 . Determination was performed by multiple reaction monitoring mode in two channels,475→100,475→283,using electrospray ionization in positive ion mode. Results Sildenafil had a good linear relationship in the range of 0. 1986-1. 986 ng with the linear regression equation being Y=27 750X+15 232 (r=0. 995 2). The average recovery was 96. 6% with RSD being 1. 8% (n=9). The limits of quantitation and detection were 0. 04 ng and 0. 01 ng,respectively. Conclusion The determination results of 10 batches of samples show that the method is sensitive and accurate and can be used as an appropriate technique to detect sildenafil in aphrodisiacs.
