Antimicrobial resistance analysis on clinically isolated Escherichia coli and detection of class I integrons
10.3969/j.issn.1671-9638.2014.09.003
- VernacularTitle:临床分离大肠埃希菌耐药性分析及I型整合子研究
- Author:
Xiaorong HUANG
;
Peichen LIU
;
Ruizhao CAI
;
Xiaoyan CHEN
;
Haoquan HUANG
;
Junyi ZHOU
- Publication Type:Journal Article
- Keywords:
integron;
Escherichia coli;
drug-resistance mechanism;
multidrug-resistance;
drug resistance,mi-crobial;
antimicrobial agent;
rational drug use
- From:
Chinese Journal of Infection Control
2014;(9):524-529
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study antimicrobial resistance of clinically isolated Escherichia coli (E.coli),the preva-lence of integrons in E.coli,and relation of integron with antimicrobial resistance of E.coli.Methods E.coli isola-ted from three hospitals of Guangdong Province from 2010 to 2012 were collected,and antimicrobial susceptibility testing was performed by Kirby-Bauer method;integrons were detected by polymerase chain reaction (PCR),and gene cassette was analyzed by sequencing.Results A total of 156 E.coli isolates were collected,antimicrobial sus-ceptibility testing showed that resistance rate of E.coli to most penicillins,cephalosporins,fluoroquinolones,amin-oglycosides and sulfonamides were over 50%;the resistance rate to antimicrobials < 10% included cefoperazone/sulbactam(0),imipenem(3.85%),cefotetan(4.35%),ertapenem(7.69%)and piperacillin /tazobactam (8.97%);The positive rate of class I integron was 57.69%(90/156);the positive rate of class I integron in multidrug-resist-ant and non-multidrug-resistant E.coli was 66.00% (66/100)and 64.71 % (22/34)respectively,the difference was not statistically different (P >0.05),but compared with sensitive E.coli (9.09%,2/22),the difference was statisti-cally different (P<0.01 ).There were nine types of integron-drug resistant gene cassettes in the variable regions, most of which contained aadA and dfrA.Conclusion Antimicrobial resistance of E.coli is serious;high incidence of class I integrons are widely found in E.coli,and is closely related with drug resistance of E.coli,class I integrons mainly mediated aminoglycosides,sulfonamides and beta-lactams resistance.
