Immunoblot observation of antigenic protein fractions in Paragonimus westermani reacting with human patients sera.
10.3347/kjp.1988.26.4.239
- Author:
Sung Hwan KIM
;
Yoon KONG
;
Suk Il KIM
;
Shin Yong KANG
;
Seung Yull CHO
- Publication Type:Original Article
- MeSH:
parasitology-helminth-trematoda;
Paragonimus westermani;
immunology;
antigen;
electrophoresis
- From:The Korean Journal of Parasitology
1988;26(4):239-243
- CountryRepublic of Korea
- Language:English
-
Abstract:
: In order to observe the antigenic fractions in saline extract of adult Paragonimus westermani, proteins in the crude extract were separated by sodium dodecyl sulfate-polyacylamide gel electrophoresis (SDS-PAGE) in reducing conditions. The separated protein fractions were transferred to nitrocellulose paper on which 20 sera from human paragonimiasis were reacted and immunoblotted. Out of 15 stained protein bands in SDS-PAGE, 7 reacted with the sera. Of 14 reacted bands, 30 kilodalton(kDa) band was the most frequently reacted (95%) and was a strong antigen. Protein bands of 23 and 46 kDa were also strong antigens. Bands of over 150 kDa, 120 kDa, 92 kDa, 86 kDa, 74 kDa, 62 kDa, 51 kDa, 32 kDa, 28 kDa, 16.5 kDa and 15.5 kDa were also reactive but their frequencies of the reaction were variable.
