Application of combined detection of anti-CCP, RF, and AKA in the diagnosis of rheumatoid arthritis
10.3760/cma.j.issn.1009-9158.2014.08.007
- VernacularTitle:抗CCP抗体、抗角蛋白抗体及类风湿因子联合检测在类风湿关节炎诊断中的应用
- Author:
Yuan ZHANG
;
Yinmei ZHANG
;
Liyan CUI
;
Shuo YANG
;
Jie ZHANG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Autoantibodies;
Peptides,cyclic;
Keratins;
Rheumatoid factor
- From:
Chinese Journal of Laboratory Medicine
2014;37(8):582-586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application of combined detection of three serum markers,anti-cyclic citrullinated peptide antibody (anti-CCP),rheumatoid factor (RF),anti-keratin antibodies (AKA),for the diagnosis of rheumatoid arthritis.Methods This was a prospective study.Serum samples were randomly obtained from 137 RA patients,265 other autoinmmune diease patients,and 111 normal controls.All of the volunteers were outpatients or inpatients of Peking University Third Hospital from January 2011 to September 2013.Levels of AKA,anti-CCP antibody,RF were measured by indirect immunofluorescence,chemiluminescence,and immune turbidimetry,respectively.The values of 3 serum biomarkers and their varied combinations for RA diagnosis were systemically assessed.The results were compared using fourfold table chi-square (x2) test.Results When using one serum marker,anti-CCP had the highest accuracy (80.39％),Youden's index (YI) (0.51),and Kappa (κ) value (0.55) for the diagnosis of RA.While using three serum markers,anti-CCP + (AKA/RF),AKA /(anti-CCP + RF),anti-CCP/(AKA + RF) and (AKA + RF)/(anti-CCP + RF)/(AKA + anti-CCP) had the highest accuracy (80.94,80.94,80.66),YI (0.51,0.51,0.50),and κ value (0.55,0.56,0.54).Conclusion AntiCCP is the vital marker for RA diagnosis.Anti-CCP + (AKA/RF),AKA/(anti-CCP + RF),and (AKA +RF)/(anti-CCP + RF)/(AKA + anti-CCP) are the most ideal combinations for RA diagnosis.
