Role of FDCs-miR-548m-CDK6 axis in clonogenicity of mantle cell lymphoma
10.3969/j.issn.1000-8179.20141149
- VernacularTitle:FDCs-miR-548m-CDK6轴在套细胞淋巴瘤集落形成中的研究
- Author:
Fang WANG
;
Xinwei ZHANG
;
Yizhuo ZHANG
;
Feng WEI
;
Xiubao REN
- Publication Type:Journal Article
- Keywords:
mantle cell lymphoma;
FDCs;
miR-548m;
CDK6;
colony formation
- From:
Chinese Journal of Clinical Oncology
2014;(18):1175-1179
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the role of FDCs-miR-548m-CDK6 axis on clonogenicity in mantle cell lymphoma. Methods:RT-qPCR and Western blot were used respectively to test the expression of miR-548m and CDK6. Bioinformatics assay was applied to predict the targets of miR-548m, and Western Blot was used to test the expression level of CDK6 after miR-548m overexpression or in-hibition. Luciferase report assay was performed to test whether CDK6 was a direct target of miR-548m. Colony forming assay was used to test the colony forming activity in MCL after overexpression of miR-548m or knockdown of CDK6. Results:Cell adhesion to FDCs induced downregulation of miR-548m and CDK6 expression in MCL. Bioinformatics assay revealed that miR-548m could target the 3'-UTR of CDK6 and that a negative correlation exists between the level of miR-548m and the CDK6 expression. Luciferase report as-say confirmed that miR-548m directly targeted 3'-UTR of CDK6. Colony forming assay showed that overexpression of miR-548m or knockdown of CDK6 significantly suppressed MCL colony formation. Conclusion:This study reveals that FDC-enhanced mantle cell lymphoma clonogenicity is mediated by the miR-548m/CDK6 axis.
