The effects of extract ofWujia-Shenghua capsule on proliferation of the MCF-7 cells
10.3760/cma.j.issn.1673-4246.2014.10.011
- VernacularTitle:五加生化胶囊提取物对人乳腺癌细胞增殖的影响
- Author:
Fashan WANG
;
Song LI
;
Tienan LI
;
Hongbin ZHU
;
Yang LI
;
Haiyang LIU
;
Ning ZHANG
- Publication Type:Journal Article
- Keywords:
Wujia-Shenghua capsule extract;
MCF-7 cell;
MTT test;
Plant estrogen-like effects;
Effective medication site
- From:
International Journal of Traditional Chinese Medicine
2014;(10):902-904
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of estrogenic-like effects ofWujia-Shenghua capsule and effective medication site.MethodsThrough D-101 macroporous resin column methods,Wujia-Shenghua capsule with 60% ethanol extraction was separated for water elution part, 20% ethanol elution part, 40% ethanol elution part, 60% ethanol elution part. Then each elution part was respectively mixed into concentration of 10mg/ml,1mg/ml, 0.1mg/ml, 0.01 mg/ml and acted on the MCF-7 cell to have, MTT test and rate of PR calculation.Results Compared with the blank control group(100%), when the concentration was 0.1 mg/ml, water elution part, 20% ethanol elution part and 60% ethanol elution part(98.10%, 101.06%, 106.04%)had no effect on the proliferation of MCF-7 cells, there was not statistically significant(P>0.05), while the 40% ethanol elution part(108.22%)can promote the proliferation of MCF-7 cells,there was a statistically significant(P<0.05). When the concentration was 1 mg/ml, 20%, 40% and 60% ethanol elution part(111.72%, 122.48%, 115.35%)can distinctly promote the proliferation of MCF-7 cells, there was a significant difference between four groups(P<0.01).ConclusionThe 40% ethanol elution part ofWujia-shenghua capsule has the strongest estrogen activity on plant.
