Determination of hesperidine inErchen mixture by HPLC
10.3760/cma.j.issn.1673-4246.2014.10.013
- VernacularTitle:HPLC测定二陈合剂中橙皮苷含量方法研究
- Author:
Jing LIU
;
Peigen HE
;
Zhihao LI
;
Peng LI
- Publication Type:Journal Article
- Keywords:
Hesperidine;
Erchen mixture;
HPLC
- From:
International Journal of Traditional Chinese Medicine
2014;(10):909-911
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of hesperidine inErchen mixture. Methods HPLC method was used with Fortis C18(250 mm×4.6 mm,5μm)column and the mobile phase consisted of acetonitrile-acetic acid-water(22∶4∶74). The flow rate was 1.0 ml/min,the column temperature was 30℃, and the UV detector was set at 283 nm.Results The linear ranges of hesperidine was 2.86-57.20μg(r=0.999 8). The average recovery of Aloin was 99.4%.Conclusion The method is rapid, reliable, simple, repeatable and with high specificity. It can be an important supplement for Erchen mixture elevated standard.
