Alterations of Treg cells, Th17 cells and some related cytokines in peripheral blood of patients with hand-foot-mouth disease
10.3760/cma.j.issn.0254-5101.2014.09.009
- VernacularTitle:Th17和 Treg 细胞及相关细胞因子在手足口病患儿外周血中的变化
- Author:
Li YANG
;
Shuyuan SUN
;
Qingxiao XUE
;
Bo LI
;
Hongwei TIAN
;
Xiaojin LIU
;
Huimin YAN
;
Erhei DAI
- Publication Type:Journal Article
- Keywords:
Hand-foot-mouth disease;
Treg cells;
Th17 cells;
Cytokines
- From:Chinese Journal of Microbiology and Immunology
2014;(9):697-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alterations of Treg cells , Th17 cells and related cyto-kines in peripheral blood of patients during the early stage of hand-foot-mouth disease ( HFMD) .Methods Flow cytometry was performed to analyze the percentages of Treg cells ( CD4+CD25+Foxp3 T cells) and Th17 cells ( CD3+CD8-IL-17+T cells) in peripheral blood samples collected from 49 patients with severe HFMD , 26 patients with common HFMD and 30 healthy children.The levels of IL-6, IL-10, IL-17, IL-23 and TGF-β1 in serum samples were measured by ELISA .Results The percentages of Treg cells , ratios of Treg/Th17 cells, serum levels of TGF-β1 and IL-10 in patients with HFMD were significantly decreased as compared with those of control group (F=5.580, 6.205, 0.000, 0.014, respectively, P<0.05).Patients with se-vere HFMD showed a significantly increased Th17 cells (F=3.189 P<0.05) and a tendency of enhanced IL-17 expression , but no significant differences with the levels of IL-17 were observed .No significant differ-ences with the expression of IL-23 in the patients among each group were detected (P>0.05).The levels of IL-6 in serum samples from severe disease group were obviously increased as compared with those of common HFMD group and control group (F=7.318, P<0.05).Conclusion The results of this study demonstrated that the levels of Treg , Th17 cells and some related cytokines were varied in peripheral blood of patients dur-ing the early stage of HFMD .Inflammatory responses were enhanced to promote anti-virus activities by sup-pressing Treg cells and stimulating Th17 cells.
