Changes and clinical significance of Th17 percentage and FOXP3 concentration in peripheral blood of children with bronchial asthma
10.3969/j.issn.1673-4130.2014.18.012
- VernacularTitle:儿童支气管哮喘患者外周血Th17百分数及FOXP3水平的变化及意义
- Author:
Qingju ZHANG
;
Fang GONG
;
Weihong SHEN
- Publication Type:Journal Article
- Keywords:
bronchial asthma;
helper T cells;
regulatory T cells;
forkhead transcription factor 3
- From:
International Journal of Laboratory Medicine
2014;(18):2451-2452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the percentage of Th17 cells and FOXP3 concentration in peripheral blood of children with bronchial asthma and their clinical significance .Methods Thirty children with bronchial asthma and thirty healthy children as con-trol group were enrolled in the study .The percentage of Th17 and CD4+CD25+ Treg cells in the peripheral blood were determined by flow cytometry(FCM) .The mRNA expression of FOXP3 in the peripheral blood was determined by quantitative real-time PCR . The concentrations of IL-17 and IL-10 in plasma were measured by using ELISA .Results In children with bronchial asthma ,the proportions of Th17 cells in the peripheral blood and concentration of IL-17 in plasma increased ,while the expression of FOXP3 in the peripheral blood and concentration of IL-10 in plasma decreased .Conclusion The imbalance of Th17/FOXP3 may contribute to the proceeding of bronchial asthma in children .
