The development and application of stabilizers for ELISA test kit
10.3969/j.issn.1673-4130.2014.18.045
- VernacularTitle:酶联免疫吸附试验试剂盒稳定剂的研制与应用
- Author:
Xiangli WU
;
Lei SHI
;
Shuo CUI
;
Jinghui YAN
- Publication Type:Journal Article
- Keywords:
enzyme-linked immunosorbent assay kit;
coating plate;
enzyme labeled antibody;
stabilizer
- From:
International Journal of Laboratory Medicine
2014;(18):2527-2528
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop ELISA kit stabilizer for each component ,with which the shelf life of kit could meet the com-mercial need .Methods A series of stabilizers for microplate ,enzyme-labeled antibody and standards were prepared by the means of experience and orthogonal approach ,through controlled trials ,screening out the best stabilizer formulations .Results A kind of coa-ted stabilizer (mass fraction :bovine serum albumin 0 .5% ,gelatin 0 .25% ,trehalose 5% ,PEG4000 0 .1% ) and a kind of enzyme la-beled antibody stabilizer (mass fraction :Bovine serum albumin 1% ,peptone 1% ,sucrose 10% ,trehalose 5% ,PEG4000 0 .25% ) were screened out .Conclusion The ELISA kits can be stable at 4 ℃ for 1 year after treated with the obtained stabilizers .
