Analysis of test results using residual reagents in electrochemiluminescence immunoassay analyzer
10.3969/j.issn.1673-4130.2014.18.046
- VernacularTitle:罗氏电化学发光免疫分析仪残留试剂的检测结果分析
- Author:
Wenli XU
;
Changzheng LIAO
;
Yanhua LIN
- Publication Type:Journal Article
- Keywords:
residual reagent;
Electrochemiluminescence immunoassay analyzer;
Result analysis
- From:
International Journal of Laboratory Medicine
2014;(18):2529-2530
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the detection efficiency of residual reagent used in electrochemiluminescence immunoassay an-alyzer .Methods Alpha fetoprotein (AFP) ,carcino-embryonic antigen(CEA) and total prostate specific antigen(TPSA) were de-tected by using remaining reagents .The precision and recovery rate of remaining reagents were analyzed .Referring to the document of NCCLS EP9-A2 ,8 clinic samples were detected everyday by residual reagent and new reagent respectively .The test results of to-tal 40 samples were recorded within 5 days .The test results derived from 2 kinds of reagents were analyzed comparatively and their bias was evaluated by using new reagent as a control method and residual reagent as experimental methods .Results The within-run and between-run coefficients of variation(CV) of the 3 items measured by residual reagents in low and high levels of quality control products met the related standard .The recovery rate was variable from 90% to 110% .The test rewults of the 2 kinds of reagents were positively correlated(r2 >0 .95) .Their anticipated biases were within allowed biases on the medical decision level of CEA ,AFP and T PSA .Conclusion Residual reagent of electrochemiluminescence immunoassay analyzer can meet the clinical practice needs , which also can ensure the quality of measurement and the reduction of the cost .
