Comparison of 3 methods in the detection for hepatitis B virus PreS 1 antigen
10.3969/j.issn.1673-4130.2014.18.041
- VernacularTitle:3种方式检测乙型肝炎病毒前S1抗原的比较研究
- Author:
Jie FU
;
Dongneng JIANG
;
Xiaoyun PU
- Publication Type:Journal Article
- Keywords:
automatic enzyme immunoassay analyzer;
time-resolved fluorescence analysis;
enzyme linked immunosorbent assay;
hepatitis B virus PreS 1 antigen
- From:
International Journal of Laboratory Medicine
2014;(18):2519-2520
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the similarities and differences of 3 methods for detecting hepatitis B virus S 1(PreS1) ,and select the appropriate method for detecting clinical samples .Methods The PreS1 of the serum samples from chronic hepatitis B pa-tients were tested with enzyme immunoassay analyzer ,time-resolved method and the manual method .To compare the repetition rate ,select PreS1 antigen strongly positive serum and weak positive serum were detected 15 times by three methods ;To compare the explicit rate ,the reaction temperature was raised or lowered by 3 ℃ .Results The positive rate of three methods was 93 .53% , 92 .81% ,92 .81% .Automated ELISA reproducibility CV strong positive CV3 .62% ,weakly positive CV was 13 .42% ,CV of time-resolved method for the 2 kinds of samples were 5 .10% ,7 .92% ,manual methods CV 11 .10% 29 .88% ;changing the reaction incu-bation temperature 3 ℃ ,automatic detection ELISA all specimens S/CO value decreased ,increasing the chance of a false negative . The manual methods and time-resolved detection method for all specimens S/CO values increased ,increasing the chance of a false positive .Conclusion The detection rate and repeatability of automated ELISA were better .The time-resolved method followed and the manual methods were poor .
