Effect of VHL on the invasion and migration of glioma U251 cells
10.3969/j.issn.1000-8179.20140584
- VernacularTitle:VHL基因对U251胶质瘤细胞侵袭和迁移的影响
- Author:
Bing XIAO
;
Minhua YE
;
Xuan ZHOU
;
Miaojing WU
;
Lei HAN
;
Chunsheng KANG
;
Xingen ZHU
- Publication Type:Journal Article
- Keywords:
VHL;
glioma;
invasion;
migration;
MMPs
- From:
Chinese Journal of Clinical Oncology
2014;(15):957-960
- CountryChina
- Language:Chinese
-
Abstract:
To determine the effects of Von Hippel-Lindau (VHL) on the invasion and migration of glioma U251 cells. Methods:U251 GBM cells were transfected using VHL expression plasmid. Real-time polymerase chain reaction was conducted to de-tect VHL mRNA expression after transfection. Western blot assay was used to measure protein (VHL, MMP-2, and MMP-9) expres-sion. Tumor invasion and migration were examined by the Transwell and wound-healing experimental methods after VHL up-regula-tion. The intracranial model of nude mouse was developed using U251 cells transfected by VHL expression plasmid, and immunohisto-chemical staining was used to measure protein (VHL, MMP-2, and MMP-9) expression in the tissue sections. Results: In the U251 cells transfected by VHL expression plasmid, the expression of VHL mRNA and VHL proteins increased, and the expression of MMP-2 and MMP-9 protein decreased. Meanwhile, the invasion and migration of glioma U251 cells were also inhibited. Immunohistochemical staining results showed that the expression of MMP-2 and MMP-9 proteins decreased, and the VHL protein expression increased after transfection. Conclusion:VHL can inhibit the invasion and migration of glioma U251 cells. Thus, VHL gene can be used as a target for the gene therapy of gliomas.
