Serum melatonin and hippocampus MT1 overexpression in asthmatic rat models
10.3969/j.issn.1001-1978.2014.08.021
- VernacularTitle:哮喘大鼠血清褪黑素及海马褪黑素受体1的变化
- Author:
Wen CHEN
;
Guanghe FEI
- Publication Type:Journal Article
- Keywords:
asthma;
rat;
melatonin;
melatonin recep-tor 1 ( MT1 );
hippocampus;
neuroimmunomodulation
- From:
Chinese Pharmacological Bulletin
2014;(8):1142-1146
- CountryChina
- Language:Chinese
-
Abstract:
Aim ToinvestigatetheexpressionofMT1 in the hippocampus and serum melatonin in the asth-matic rats, and explore the mechanism in the develop-mentofasthma.Methods SixtyadultSDratswere randomly divided into two groups: control group ( n=20 ) and asthma group ( n=40 ) . Asthma rat model was established by sensitization and stimulation with ovalbumin ( OVA ) . Immunohistochemistry, Western blot, and reverse transcription PCR ( RT-PCR ) were used to evaluate the expression of MT1 in hippocam-pus. Enzyme linked immunosorbent assay ( ELISA ) was used to detect serum melatonin level. Results TheexpressionofMT1inhippocampusatgeneand protein levels were significantly elevated in asthmatic group ( P <0. 05 ) compared with the control group,whereas serum melatonin was obviously reduced ( P<0. 05 ) . Compared with day 5 and day 10 in the asth-matic group, there was a significant increase of MT1 in hippocampus on day 17 ( P<0. 05 ) , while there was no significant difference between day 5 and day 10 ( P>0.05).Conclusions MelatoninandMT1maybe involved in the pathogenesis of asthma. The up-regula-tion of MT1 in hippocampus with time-dependent pat-tern may be a compensatory response to decreased pe-ripheral melatonin levels for augmenting melatoninˊs neuroprotective and neuroimmunomodulatory effects a-gainst inflammatory reaction and stress in asthma.
