Comparison of two asthma models in rats
10.3969/j.issn.1001-1978.2014.08.028
- VernacularTitle:两种大鼠哮喘模型的比较
- Author:
Yajuan WANG
;
Yiting ZHAO
;
Bin DAI
;
Huifang TANG
;
Yali JIANG
;
Xuefeng WANG
;
Jiqiang CHEN
- Publication Type:Journal Article
- Keywords:
asthma;
OVA;
pertussis;
bronchial hyperreactivi-ty;
inflammation;
rats
- From:
Chinese Pharmacological Bulletin
2014;(8):1175-1178
- CountryChina
- Language:Chinese
-
Abstract:
Aim Toestablishanexcellentratasthmamodel from using OVA+pertussis sensitized, OVA sensitized and per-tussissensitizedrats.Methods Thethreemethodswereusedto sensitize rats;methacholine bronchial provocation tests were per-formed to determine airway hyperresponsiveness;bronchoalveolar lavage fluid ( BALF) was prepared after the animals were chal-lenged by nebulized antigen. The differential white cell count in BALF was performed, and lung tissue was detected by morpho-logicalanalysis.Results AllofOVA+pertussissensitization,OVA sensitization and pertussis sensitization could deteriorate lung function, increase inflammatory cells and cause pathological change, and OVA + pertussis sensitized rat model had better effect than OVA sensitized and pertussis sensitized rat models. Conclusion OVA+pertussissensitizationandOVAaerosolisa successful rat asthma model.
