Establishment of eosinophilic bronchitis mice model without airway hyperresponsiveness
10.3760/cma.j.issn.1008-1372.2014.07.007
- VernacularTitle:无气道高反应性的嗜酸粒细胞性支气管炎小鼠模型的建立
- Author:
Jiaxing XIE
;
Qingling ZHANG
;
Liyan CHEN
;
Wei LUO
;
Kefang LAI
;
Nanshan ZHONG
- Publication Type:Journal Article
- Keywords:
Asthma;
Eosinophils;
Bronchitis;
Disease models,animal;
Bronchial hyperreactivity;
Mice
- From:
Journal of Chinese Physician
2014;(7):882-885
- CountryChina
- Language:Chinese
-
Abstract:
Objectiev To explore the use of different nebulizer to establish mice model that have airway eosinophilic inflam -mation without airway hyperresponsiveness .Methods Female BALB/c mice were obtained and divided randomly into 3 groups:eo-sinophilic airway inflammation group ( experimental group ) , asthma group, and control group .Mice were immunized with ovalbumin ( OVA) .The experiment group and asthma group were challenged with an aerosol of 1% w/v OVA using a PARI TIA and PARI LC STAR nebulizer on day 28, 29, 30, respectively.The control mice were received saline sensitization and challenge .Airway respon-siveness was measured .Cell different counts in bronchial alveolus lavage fluid ( BALF) were performed and a pathologist performed histopathological evaluation of the trachea and lung .Results Airway responsiveness in the experimental group was not significantly different compared with the normal saline ( NS) group but was significantly different compared with the asthma group .Eosinophils in BALF were increased significantly in experimental group compared with the NS group , and significant difference was observed between experimental group and asthma group .The intensity of airway inflammation in experimental group was milder than that in the asthma model .Conclusions We established an eosinophilic bronchitis mice model without hyperresponsiveness successfully .Our model es-tablished a foundation for the further research in airway hyperresponsiveness .
