Study on inhibitory effect of baicalein on mycoplasma pneumonia and protection mechanism of pulmonary epithelial cells of mice
10.3760/cma.j.issn.1008-1372.2014.07.018
- VernacularTitle:黄芩素对肺炎支原体的抑制作用及对小鼠肺上皮细胞的保护机制研究
- Author:
Hongxia YUAN
;
Hongliang CHEN
;
Guozhi DAI
;
Anwen ZHOU
;
Yufeng PENG
- Publication Type:Journal Article
- Keywords:
BAICALIN/pharmacology;
Pneumonia,mycoplasma/drug therapy;
Lung;
Epithelial cells;
Mice,inbred BALB C
- From:
Journal of Chinese Physician
2014;(7):919-922
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of baicalein on mycoplasma pneumonia and its the protective mecha -nism in body , and to provide scientific experimental basis for prevention and treatment of mycoplasma pneumoniae infection .Methods The mycoplasma pneumoniae and baicalein treated BALB /c mice lung tissues were stained with hematoxylin eosin (HE), and his-topathological grading .Minimum inhibitory concentration ( MIC) of baicalein on mycoplasma pneumonia was determined by real-time quantitative polymerase chain reaction ( PCR) .The expression of P 1 adhesion molecules mRNA and protein in lung tissue of BALB /c mice was determined with reverse transcription-PCR and Western blot .The expression of epidermal growth factor ( EGF) mRNA and protein in lung tissue was detected by quantitative RT-PCR and immunofluorescence .Results Baicalein significantly reduced the my-coplasma treated mice's lung tissue pathological score .The minimal inhibitory concentration of baicalein was 32 μg/ml.Baicalein sig-nificantly downregulated P 1 gene transcription and protein translation , and upregulated EGF gene transcription and protein expression . Conclusions Baicalein shows significant resistance to mycoplasma pneumoniae , and can protect the body against mycoplasma damage by inhibiting the expression of P 1 protein and promoting the expression of EGF protein .
