Neurotoxicity induced by methamphetamine and the protective role of nNOS inhibitor in rats
10.3969/j.issn.1001-1978.2014.08.013
- VernacularTitle:甲基苯丙胺所致的大鼠神经毒性损伤及nNOS抑制剂的保护作用
- Author:
Jingtao XU
;
Fu ZHANG
;
Xingyi YANG
;
Weibing XIE
;
Lizeng LI
;
Chao LIU
;
Huijun WANG
- Publication Type:Journal Article
- Keywords:
methamphetamine;
neurotoxicity;
nNOS;
7-nitroindazole;
oxidative stress;
apoptosis
- From:
Chinese Pharmacological Bulletin
2014;(8):1101-1106
- CountryChina
- Language:Chinese
-
Abstract:
Aim Tostudytheprotectiveeffectsofin-hibition of neuronal nitric oxide synthase ( nNOS ) in methamphetamine ( METH ) induced neurotoxicity via oxidativestressinjuryinrats.Methods Ratmodelsof acute METH poisoning with/without 7 nitroindazole (7-NI) pretreatment were built to evaluate the protec-tive effects on the changes of ethology, nNOS, nitro-proteins, dopamine ( DA ) and apoptosis in rat stria-tum.Results ThenNOSexpression,nitroproteinex-pression and apoptosis increased significantly in the METH group( P<0. 05 ) and 7-NI pretreatment shows a dramatically protective effect against the changes ( P<0. 05 ) . DA concentration decreased significantly in the METH group(P<0. 05) and 7-NI pretreatment re-stored the depleted DA to normal level ( P >0. 05 ) . Stereotyped behavior increased significantly in the METH group and the 7-NI combined group compared with the 7-NI group and the control group(P<0. 01). Conclusions 7-NIshowssignificantprotectiveeffects against the alterations of DA level, nNOS expression, nitroprotein expression and apoptosis in rat striatum caused by acute METH poisoning. However, there is no obvious protective effect on METH-induced stereo-typed behavior.
