Expression and significance of programmed death-1 on T cells of patients with drug-sensitive or multiple drug-resistant tuberculosis
10.3760/cma.j.issn.1000-6680.2014.07.008
- VernacularTitle:程序性死亡分子1在药物敏感及耐多药肺结核患者T淋巴细胞上的表达及意义
- Author:
Changzhen HU
;
Xingliu WU
;
Xiaohua CHEN
;
Xiaoping TANG
- Publication Type:Journal Article
- Keywords:
Tuberculosis,pulmonary;
PD-1;
T-lymphocytes
- From:
Chinese Journal of Infectious Diseases
2014;(7):30-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of programmed death-1 (PD-1) in cellular immune response of patients with tuberculosis (TB) infection .Methods Twenty drug-sensitive (DS) and fifteen drug-resistant (DR ) subjects with TB infection who were hospitalized in Jiangmen Central Hospital affiliated Sun Yat-Sen University and Jiangmen tuberculosis dispensary from July 2011 to July 2012 were included in this study . Twenty-six healthy subjects were included as control . The expressions of PD-1 on CD4 + and CD8 + T lymphocytes were measured using flow cytometry and plasma interferon-γ (IFN-γ) ,levels were analyzed before and after TB treatment for 3 months .Kruskal-Wallis H test was used for analysis of multiple independent samples and Wilcoxon signed-rank test was used for analysis of paired samples . Pearson test was used for correlation analysis . Results After anti-TB chemotherapy ,the expressions of PD-1 on CD4 + and CD8 + T cells in both TB groups were significantly decreased (DS group :15 .99% vs 21 .59% and 11 .86% vs 18 .52% ;DR group :26 .64% vs 35 .47% and 29 .64% vs 34 .56% ) (both P < 0 .05) .Plasma IFN-γ level in DS group increased significantly after treatment(50 .65 ng/L vs 32 .31 ng/L , P < 0 .01) ,while that in DR group did not increase significantly (22 .26 ng/L vs 20 .03 ng/L ,P> 0 .05) .In DS group ,the expression of PD-1 on CD4 + T lymphocytes was negative correlated with plasma IFN-γ level both before and after anti-TB chemotherapy (pre-treatment : r = - 0 .510 , P < 0 .05 ; post-treatment : r = - 0 .520 , P< 0 .05 ) . Conclusions PD-1 modulates T cell function of immune response against TB infection in DS-TB subjects and plays a key regulatory role in CD4 + T lymphocyte mediated immunity during TB infection . However , the immunomodulatory mechanism of cell immunity in DR-TB subjects may be more complex .
