The effects of gossypol acetic acid on DNA methylation level and the mRNA expression of hMLH1 gene in human tongue carcinoma Tca8 1 1 3 cells

VernacularTitle:醋酸棉酚对人舌鳞癌 Tca8113细胞hMLH1基因甲基化和mRNA表达的影响
Author: Wenfei CHEN ; Shuai FU ; Wanyuan YUE ; Yong WU
Publication Type:Journal Article
Keywords: Human tongue carcinoma; Gossypol; Tumor suppressor gene; DNA methylation
From: Journal of Practical Stomatology 2014;(4):551-555
CountryChina
Language:Chinese
Abstract: Objective:To study the effects of gossypol acetic acid(GAA)on the methylation level and the mRNA expression of hM-LH1 in human tongue cancer Tca8113 cells.Methods:Tca8113 cells were treated by GAA at various doses for 24 h,48 h and 72 h respectively.MTT assay was used to detect the cell proliferation.Nested methylation specific PCR(nMSP)was used to detect methyl-ation level of hMLH1 .Real-time fluorescence quantitative PCR(RFQ-PCR)was applied to investigate the mRNA expression of hM-LH1 gene.Results:GAA inhibited the proliferation of Tca8113 cells dose-and-time dependently,decreased the DNA methylation level of hMLH1(P<0.05)and increased hMLH1 mRNA expression in Tca8113 cells(P<0.05).Conclusion:GAA can suppress proliferation of Tca8113 cells by demethylation of hMLH1 gene and increase of hMLH1 mRNA expression.