Expression and significance of glutathione S-transferase mu 3 in prostate cancer
10.3760/cma.j.issn.1000-6702.2014.07.011
- VernacularTitle:谷胱甘肽硫基转移酶μ-3在前列腺癌中的表达及意义
- Author:
Jianguo ZHU
;
Weihong CHEN
;
Shuxiong XU
;
Yuanlin WANG
;
Zhaolin SUN
;
Huichan HE
;
Funeng JIANG
;
Weide ZHONG
- Publication Type:Journal Article
- Keywords:
Glutathione S-transferase mu 3;
Prostate cancer;
Two-dimensional fluorescence difference gel electrophoresis
- From:
Chinese Journal of Urology
2014;(7):511-513
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role and clinical significance of GSTM 3 ( glutathione S-trans-ferase mu 3) expression in prostate cancer (PCa). Methods We had used the two-dimensional fluores-cence difference gel electrophoresis ( 2D-DIGE) and mass spectral analysis to further verify the microarray data of mRNA expression profiling discovered .GSTM3 mRNA level was detected by Rael-time Quantitative PCR ( RT-QPCR) in 28 pairs of prostate cancer tissue and benign tissue .The relationship of GSTM 3 level with the serum PSA level and the clinical feature of PCa were analyzed . Results In 2D-DIGE study, we found that the expression of GSTM 3 protein in adjacent tissues was significantly higher than that in PCa tis-sues (P<0.05).RT-QPCR results showed that GSTM3 in adjacent tissues (8.12±0.51) was significantly higher than that in PCa tissues (7.18±0.54) (P<0.05).There was no significant difference of GSTM3 ex-pression in different serum PSA packets ( P>0.05) and prostate cancer clinical pathological parameters ( P>0.05). Conclusions GSTM3 expression is down-regulated in PCa tissues, and we may identify PCa by detecting the GSTM 3 expression .
