The effects of IL-24 on the differentiation and function of osteoclasts

VernacularTitle:白细胞介素-24对破骨细胞功能及分化的影响
Author: Xin WANG ; Ce SHI ; Rong ZHENG ; Xuan LIU ; Hongchen SUN ; Lipeng WU
Publication Type:Journal Article
Keywords: Osteoclasts; RAW264.7 cell line; Bone resorption; Interleukin-24
From: Journal of Practical Stomatology 2014;(4):456-459
CountryChina
Language:Chinese
Abstract: Objective:To study the effect of IL-24 on the differentiation and function of osteoclasts.Methods:Mature osteoclasts were isolated from long bones of neonate rats.Optimal multiplicity of infection(MOI)of AdCMV-EGFP was determined.Then osteo-clasts and RAW264.7 cells were transfected with AdCMV-EGFP and AdCMV-IL-24 respectively.The function of osteoblasts was studied by the observation of bone resorption lacunae,the differentiation of RAW264.7 cells was evaluated by the expression of osteo-clast related genes examined with real-time PCR.Results:Osteoclasts were TRAP positive with more than 2 neclei.MOI=400 was suitable for AdCMV-EGFP transfection.The resorption lacunae area in AdCMV-IL24 transfected cells was larger than that in AdCMV-EGFP transfected cells(P<0.05).Real-time PCR showed that under induced conditions,osteoclastic related genes NFATc1,CTSK and TRAP were changed in RAW264.7 cells transfected with AdCMV-IL-24.Conclusion:IL-24 may promote the differentiation and function of osteoclasts.