Quantification of tryptase and TIM-3 double positive mast cells in human chronic periodontitis tissue

VernacularTitle:类胰蛋白酶和TI M-3双阳性肥大细胞在人不同程度牙周炎组织中的量化研究
Author: Juan LI ; Hua JIN ; Tian LAN ; Shiguang HUANG
Publication Type:Journal Article
Keywords: Chronic periodontitis; Mast cell; Tryptase; TIM-3
From: Journal of Practical Stomatology 2014;(4):482-486
CountryChina
Language:Chinese
Abstract: Objective:To quantify the tryptase and T cell immunoglobulin mucin domain-3(TIM-3)double positive mast cells in hu-man chronic periodontitis tissue using double immunofluorescence staining.Methods:25 healthy controls,28 chronic mild periodontitis and 30 chronic advanced periodontitis patients were included.The gingival specimens were stained with HE for histology,and with double immunofluorescence staining for the identification of tryptase and TIM-3 double positive mast cells in gingival tissue.Results:In chronic periodontitis tissue the degree of gingival inflammation was significantly increased,the densities(cells/mm2 )of tryptase and TIM-3 double positive mast cells were significantly increased(P<0.05),in addition,that in chronic advanced periodontitis group was significantly higher than in the chronic mild periodontitis group(P<0.05).Conclusion:Tryptase and TIM-3 double positive mast cells has the similar tendency as the severity of periodontitis inflammation in human periodontitis tissue.Tryptase and TIM-3 double positive mast cells may play an important role in human chronic periodontitis.