The effects of periodontitis on the expression of apoptosis-related proteins in pancreas of rats with Type 2 Diabetes Mellitus
10.3969/j.issn.1001-3733.2014.04.04
- VernacularTitle:牙周炎对Ⅱ型糖尿病大鼠胰岛细胞凋亡相关蛋白表达的影响
- Author:
Jiayi WU
;
Zhe XU
;
Jia NI
;
Dan WANG
;
Shigao LUO
;
Xi YANG
;
Dongying XUAN
;
Jincai ZHANG
- Publication Type:Journal Article
- Keywords:
Periodontitis;
Type 2 Diabetes Mellitus;
Apoptosis-related proteins
- From:
Journal of Practical Stomatology
2014;(4):464-468
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of periodontitis on the expression of apoptosis-related proteins in pancreas of rats with Type 2 Diabetes Mellitus.Methods:Spontaneously type 2 diabetic OLETF rats were randomly divided into 2 groups:diabetes with or without periodontitis(diabetes group and combination group).LETO rats with the same germline and the same age but having normal glucose tolerance were randomly divided into control group and periodontitis group.20 weeks after periodontitis were established,all the rats were sacrificed and the pancreas were pathologically examined by HE staining.The expression of Bax,Bcl-2 and Caspase-3 in the pancreas islet were detected by immunohistochemistry staining and semi-quantitative analysis.Results:The expression of Bax, Bcl-2 and Caspase-3 in the pancreas islet was no significant difference between control and periodontitis groups(P=0.324,P=0.091,P=0.852).Compared with diabetes group,the expression of Bax and Caspase-3 in combination group showed a significant increase(P=0.000,P=0.000),and the expression of Bcl-2 was significantly decreased(P=0.022).Conclusion:Under healthy conditions,periodontitis has no effect on the expression of apoptosis-related proteins in rat pancreas islet.However,in rats with diabe-tes,periodontitis may affect the expression of apoptosis-related proteins in pancreas islet.
