Preparation of transferrin and RGD co-modified PLGA nanoparticles and its targeting to melanoma
- VernacularTitle:转铁蛋白与RGD共修饰PLGA纳米粒的制备及其对黑色素瘤的靶向性研究
- Author:
Zongxiang LI
;
Ping SUN
- Publication Type:Journal Article
- Keywords:
transferrin;
RGD;
PLGA nanoparticle;
melanoma;
drug targeting
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;37(4):19-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare transferrin and Arg-Gly-Asp polypeptide co-modified nanoparticles(TF/RGD-NPs)and evaluate its targeting efficiency to melanoma.Methods The co-modified nanoparticles were prepared by emulsion method and its appearance,particle size and Zeta potential were evaluated.The cellular uptake experiment and melanoma tumor spheroids penetration test were used to evaluate the affinity and ability to penetrate tumor tissues of TF/RGD-NPs to melanoma B16 cells. Results The particle diameter of co-modified nanoparticles was(113.4 ±12.5)nm and the Zeta potential was(4.53 ±2.15)mV.In vitro uptake test demonstrated that the efficacy of cellular uptaken TF/RGD-NPs by B16 cells were 2.7 times and 2.9 times to TF-NPs and RGD-NPs,respectively,the differences were all significant(P<0.05 ).Tumor spheroid penetration test results showed that TF/RGD-NPs has good affinity to melanoma cells.Conclusion TF/RGD-NPs can target to melanoma B16 cell efficiency in vitro,it may be serve as a potential drug delivery system for targeting melanoma.