Dicer knockdown enhance the proliferation, migration and invasion of cholangiocarcinoma cell line TFK-1

VernacularTitle:抑制Dicer基因对胆管癌细胞株TFK-1增殖、迁移和侵袭的影响
Author: Li TIAN ; Wenlong CHENG ; Yongjun CHEN
Publication Type:Journal Article
Keywords: Gene,Dicer; Cholangiocarcinoma cell; Proliferation; Migration; Invasion
From: Chinese Journal of Hepatobiliary Surgery 2014;20(7):524-527
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of Dicer knockdown on the proliferation,migration and invasion of cholangiocarcinoma cell line TFK-1.Methods The study used liposome-mediated method to transfect Dicer-siRNA into TFK-1 cells.The mRNA and protein expressions of Dicer after transfection were detected by real-time PCR and western blot.CCK8 assay,scratch test and transwell assays were performed to analyze the influence on the proliferation,migration and invasion of cells,respectively.Results Compared with the control,Dicer mRNA level at 48 h and Dicer protein level at 96 h were both significantly reduced after transfection with Dicer-siRNA (P ＜ 0.01).CCK8 assay revealed that the absorbance value significantly increased at 72 h,96 h and 120 h after transfection (P ＜0.01).Scratch test and transwell assay showed that the migration ability and the number of invasive cells significantly increased (P ＜ 0.05).Conclusion Dicer knockdown enhanced the proliferation,migration and invasion of cholangiocarcinoma cell line TFK-1.