High Throughput Screening of Drug Multi Residues in Fish by Quadrupole-Time-of-Flight Mass Spectrometry
10.11895/j.issn.0253-3820.140322
- VernacularTitle:四级杆飞行时间串联质谱高通量筛查鱼肉中的药物残留
- Author:
Qing LI
;
Huitai LUO
;
Xiaolan HUANG
;
Zhixin ZHU
;
Huiqin WU
- Publication Type:Journal Article
- Keywords:
Quadrupole-time-of-flight mass spectrometry;
Tetracycliens;
Sulfonamides;
Quinolones;
Triphenylmethanes;
Estrogens;
Androgens;
Glucocorticoids;
High throughput screening
- From:
Chinese Journal of Analytical Chemistry
2014;(10):1478-1485
- CountryChina
- Language:Chinese
-
Abstract:
A high-throughput method for screening drug multi residues was developed by quadrupole-time-of-flight( Q-TOF) mass spectrometry together with QuEChERS sample preparation technique. It has been used for the determination of 57 drugs, such as tetracyclines ( TCs ) , sulfonamides ( SAs ) , quinolones ( QNs ) , triphenylmethanes (TPMs), estrogens (ETs), androgens (AGs) and glucocorticoids (GCs) in fish. The optimized pretreatment conditions were examined. The target compounds were extracted with acetonitrile under the condition of Na2 EDTA-Mcllvaine buffer, and the usage of clean-up reagents was 25 mg anhydrous magnesium sulfate, 12. 5 mg octadecylsilane and 6. 25 mg N-Propylethylenediamine sorbent for extracted solvent of each milliliter. The positive results acquired by this high-throughput method were confirmed by liquid chromatography-tandem mass spectrometry ( LC-MS/MS) . The detection limit for these 59 drugs was in the range of 0. 5-5. 3 μg/kg. The method is time-saving, convenient, effective and wide coverage. Its sensitivity can meet the requirement of the detection of drug residues in aquatic products.
