Determination of Alkaloids in Honey by Dispersive Solid Phase Extraction and High Performance Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.140350
- VernacularTitle:分散固相萃取-高效液相色谱-串联质谱法测定蜂蜜中生物碱
- Author:
Weihua GUO
;
Jinhui ZHOU
;
Jingping HUANG
;
Peng WANG
;
Yi LI
- Publication Type:Journal Article
- Keywords:
Dispersive solid phase extraction;
High performance liquid chromatography-tandem mass spectrometry;
Pyrrolizidine alkaloids;
Isoquinoline alkaloids;
Honey
- From:
Chinese Journal of Analytical Chemistry
2014;(10):1453-1458
- CountryChina
- Language:Chinese
-
Abstract:
A method was developed for simultaneous determination of pyrrolizidine alkaloids and isoquinoline alkaloids in honey by dispersive solid phase extraction and high performance liquid chromatography-tandem mass spectrometry ( QuEChERS-HPLC-MS/MS) . The honey samples were extracted with acetonitrile solution and cleaned up with PSA absorbent. Agilent Poroshell 120 SB-C18 chromatographic column was used to separate alkaloids with high sensitivity and satisfactory resolution. The identification and quantification were achieved by using electrospray ionization in positive ion mode ( ESI+) with multiple reaction monitoring (MRM). Matrix-matched calibration curves with good correlation coefficients (R2>0. 99) were obtained in the concentration range of 0. 1-100 μg/L. The recoveries of the spiked samples at 1-100 μg/kg were in the range of 70% to 110% with the RSD of intra-day and inter-day lower than 15% and 20%, respectively. The limits of detection ( LOD) and limits of quantification ( LOQ) for all alkaloids were 0. 3 and 1. 0 μg/kg, respectively. This method was successfully applied to the simultaneous determination of pyrrolizidine alkaloids and isoquinoline alkaloids for quantification and confirmation in honey samples.
