Determination of K+, Na+and Ca2+in Miorodialysis of Apoplast of Poplar Stems by Atomic Absorption Spectrometry with Microdialysis Method
10.11895/j.issn.0253-3820.140504
- VernacularTitle:杨树嫩茎质外体微透析液中K+，Na+，Ca2+的分析
- Author:
Jinlong MA
;
Guobin JIANG
;
Shanjing YAO
;
Bo JIANG
;
Hua JIN
- Publication Type:Journal Article
- Keywords:
Poplar;
Apoplast;
Microdialysis;
Metal ions
- From:
Chinese Journal of Analytical Chemistry
2014;(10):1535-1538
- CountryChina
- Language:Chinese
-
Abstract:
The dialysis for three species of poplar stems apoplast was obtained by microdialysis method, and then the concentration of Na+, K+, Ca2+ in the dialysis sample was directly measured by graphite furnace atomic absorption spectrometry ( GF-AAS) . The flow rate of perfusate was 1μL/min and the cut-off molecular weight of probe was 30 kDa. The real-time and non-destructive test technique established for the detection of ion concentration could be applied in research of poplar biochemical physiology. The recovery of the proposed method was 95. 8%-103. 1%. The contents of sodium ion in Populus wutunensis, Populus simonii and Salix matsudana were 1034-1156 μg/L, 1493-1611 μg/L and 1586-1703 μg/L; potassium ion were 1012-1237 μg/L, 941-964 μg/L and 1095-1201 μg/L; calcium ion were 4976-5237 μg/L, 4786-5042 μg/L and 5893-6142 μg/L. The method could provide reliable data for the study of ion concentration changes of plant response to adapt to all kinds of external environment.
